Proteomics
Southern blotting
Western blotting
Northern blotting
GFP (Green Fluorescent Protein)
DNA Isolation System
RNA Isolation System
DNA Extraction from Whole Blood
DNA Extraction from Tissue
RNA Extraction from Cultured Cell
RNA Extraction from Tissue
Proteomics
This is the new field emerged in the post-genome era. The proteomics includes the research area
of proteins expressed from genes or proteins in general. The 2D electrophoresis followed by image
analysis and further qualitatively analysed by mass spectrometry is the general strategy towards
proteomics accepted by many researchers now.
Recommended System
FLA-5100
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Southern blotting
A very widely known method for the detection of a specific sequence in DNA. This method was named
after Dr. E. M. Southern who introduced this method in 1975. After the gel electrophoresis of the
sample DNA, blotting to a membrane is done and DNAs are transcribed to the membrane. A DNA probe
of specific sequence is hybridized with the membrane. When the probe is a radioisotope (RI),
exposure to phosphor imaging plate (IP) and detection by BAS system is done. When the probe is
digoxigenine (DIG), a sandwich method using enzyme (ALP or HRP) tagged anti-DIG-antibody and
chemilminescent detection by using chemiluminescence substrate is done. High sensitive detection
of chemiluminescence is effectively done by a cooled CCD camera system.
Recommended System
LAS-3000
FLA-5100
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Western blotting
A widely used method for the detection of specific protein after electrophoresis. The sample is
electrophoresed on a polyacrylamide gel, then, blotted to a membrane. The membrane is incubated with
the antibody to the specific protein. Further, the secondary antibody labeled with an enzyme such as
HRP (or ALP) is added and reacted. Chemiluminescence detection of the specific protein can be done
by applying chemiluminescent substrate of the enzyme.
Recommended System
LAS-3000
FLA-5100
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Northern blotting
A widely used detection method for a specific RNA after electrophoresis. The electrophoresis gel is
blotted to a membrane and the transcribed RNA is detected by hybridization with a labeled cDNA probe.
Phosphor imaging plate (IP) is used for the detection of RI probes, and very high sensitive cooled CCD
camera system is used for detection of chemiluminescent probes. The amount of RNA in a cell is much
less than DNA, and RNA is easily degraded by RNAse existing elsewhere. So, the handling of RNA must be
done under extreme care. Because we can get molecular weight information from the electrophoresis
length, Northern blotting is useful for verification purposes. This is the merit of Northern blotting
compared to other methods such as microarray method.
Recommended System
FLA-5100
LAS-3000
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GFP
(Green Fluorescent Protein)
A new tool inevitable for visualizing various processes of metabolism in a cell. The origin of GFP was
from a jelly fish (Aequorea victoria). A green fluorescence appeared after excitation of blue light.
Various variants are available now. Fluorescent scanner or CCD camera system with appropriate excitation
light source can be used for the imaging of GFP.
Recommended System
QuickGene-810
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DNA Isolation System
For Genetic test and Genetic study, it is required to extract high quality of DNA quickly. An automated
instrument and the kits can isolate DNA from samples easily and rapidly.
Recommended System
QuickGene-810
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RNA Isolation System
To analyse the amount of gene expression in order to investigate protein expression or drug discovery, RNA
extraction should be completed in short time. An automated extraction system can isolate RNA with less
contamination quickly.
Recommended System
QuickGene-810
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DNA Extraction from Whole Blood
Genomic DNA can be isolated from nucleus of peripheral blood cells. Genomic DNA isolated from blood
samples are used for genomic assays or genotyping studies.
Recommended System
QuickGene-810
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DNA Extraction from Tissue
To check the individual genetic characteristics or to find the position of mutagenesis, genomic DNA
is extracted from a small piece of tail or organ.
Recommended System
QuickGene-810
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RNA Extraction from Cultured Cell
Real Time PCR is one of the important technologies to measure the expression level of RNA that
affects the cell's response to drugs.
Recommended System
QuickGene-810
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RNA Extraction from Tissue
Gene expression studies, such as drag metabolism and cancer analysis, require efficient extraction
methods of high quality RNA. Isolation of total RNA from small samples of variety tissues by traditional
methods can be difficult and inefficient. Automated system is suitable for RNA isolation to avoid RNase
contamination and to get the best yield and quality in steady.
Recommended System
QuickGene-810
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