The new FLA-5100 imaging system allows imaging of fluorescent and radioisotopic samples as large as 40 x 46cm at pixel size as low as 10 microns. This system is especially suitable for protein electrophoresis in the proteomics field and general array analysis for genomics.

FEATURES AND BENEFITS

Large scanning size

Large scanning size (up to 40 x 46cm) with selectable scanning area is especially suitable for large size 2D protein electrophoresis gel analysis. Available imaging plates (IPs) include: 1 x (3543 MS IP), 2 x (2340, 2325 MS IP) and 2 x (2040, 2025 MS, SR, ND, TR IP). Scanning pixel sizes are user-selectable at 10, 25, 50, 100 or 200-micron pixels depending on specific application requirements.

Up to 4 lasers

Multiple lasers add imaging applications. The FLA-5100 includes three standard lasers: SHG blue laser (473nm), SHG green laser (532nm) and LD red laser (635nm). A fourth - LD laser for far-red (670nm) is available as an option.

Versatile

The versatility of multi-format detection allows many sampling opportunities with a single sampling tool. Radioisotope by IP method: IP-S (standard mode for RI detection) generates logarithmic converted values in PSL units. IP-V (variable mode) can change the high voltage of the photomultiplier tube (PMT), which enables X-ray diffraction study, non- destructive testing and other IP detection studies. Fluorescence by laser scanning and PMT: Image reader software controls repetitive scanning of the sample by different laser and emission filters. When added with the second channel PMT, a laser and dual emission filter mode can be enabled. Chemiluminescence by direct detection: Chemiluminescence can be detected. Digitizing Function by Epi-illumination with fluorescent screen: Applicable for transparent gels with silver stain, CBB stain, NBT stain and others. Modular optional components provide future upgrades to meet individual application needs.

IMAGE DATA

DNA stained with SYBR® Green I after electrophoresis with agarose gel (473nm excitation laser with LPB filter).

Silver stained 2D gel of Sake yeast. Digitizing function of FLA-5000 at 250V. Data courtesy of Mr. Yoshihiro Yamamoto, Kyoto Municipal Institute for Industrial Research.

SYPRO Ruby stained 2D gel of Sake yeast. Excitation at 473nm, filter LPG. Data courtesy of Mr. Yoshihiro Yamamoto, Kyoto Municipal Institute for Industrial Research.

SYPRO Orange stained 2D gel of Sake yeast. Excitation at 473nm, filter LPB. Data courtesy of Mr. Yoshihiro Yamamoto, Kyoto Municipal Institute for Industrial Research.

CBB stained 2D gel of Sake yeast. Digitizing function of FLA-5000 at 250V. Data courtesy of Mr. Yoshihiro Yamamoto, Kyoto Municipal Institute for Industrial Research.

RI detection by BAS-MS IP. Rat whole body auto radiography of ¹⁴C-glucose.

Macroarray using Clontech's Atlas™Array membrane.